產品介紹
NCM Universal Antibody Diluent(通用型抗體稀釋液)是一種即用型的,適合于稀釋所 有免疫抗體測定(Immunofluorescence, IHC, ELISA, WB)實驗中的抗體。該試劑可用于一抗 或二抗的稀釋和配制,在4℃保存和使用不少于六個月;稀釋液中加入了多種抗體結合反應 增強劑及穩定劑,增強免疫反應,減少非特異性結合,獲得更佳的效果,可反復使用,節約 寶貴的抗體。試劑中不含有動物源的蛋白、磷酸鹽、疊氮鈉或硫柳汞類防腐劑,適合于所有 類型抗體稀釋,包括 HRP,AP 標記的抗體。
產品特色
? 即用型稀釋液,無需配制
? 適用所有抗體稀釋(IF、IHC、ELISA、WB)
? 加入抗體結合反應增強劑,效果更佳
? 不含 BSA、脫脂奶粉等動物源蛋白
? 不含磷酸鹽、疊氮鈉或硫柳汞類防腐劑
操作步驟
根據抗體使用說明書,直接用本品對一抗或二抗進行適當倍數的稀釋。保存在 NCM Universal Antibody Diluent 稀釋液中的抗體可回收于 4℃保存,至少可反復多次使用 長達 6 個月以上,直至不能達到理想結果后丟棄。
保存條件
4℃保存,冰袋運輸,至少 2 年有效;
注意事項
1. 孵育抗體時盡量在 4 oC 進行,可延長稀釋后抗體的使用壽命; 2. 本品不含疊氮鈉或硫柳汞類防腐劑,但含安全性更高的防腐劑,還需小心操作避免皮膚 直接接觸。
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