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超敏ECL化學發光試劑盒

NcmECL Ultra

貨號:
P10100
規格:
A液60ml;B液60ml
目錄價:
¥549.00
購買數量
+ -

產品介紹 

NcmECL Ultra是新賽美生物科技有限公司研發的超敏ECLEnhanced Chemiluminescent) 化學發光試劑,用于檢測 HRP(辣根過氧化物酶)標記的目的條帶,檢測靈敏度最高可達飛 克(fg)級。NcmECL Ultra 優化了底物的成分,應用了新型的 Enhancer 和氧化劑,發光強度 更高,穩定性更好,不含有毒試劑,安全性更高。 

產品特色

? 高靈敏度:最高可達飛克級別,10-15 g ? 安全性高:試劑不含對人體健康有毒的化學物質 ? 信號持續時間長:發光時間長達 30 分鐘以上 ? 穩定性高:4℃保存長達一年以上,室溫運輸穩定性好 ? 經濟節約:較同類進口試劑花費更少,所需抗體少,抗體稀釋度高 

操作步驟

1. 準備工作稀釋液,將 NcmECL Ultra Luminol/Enhancer Reagent (A) 和 NcmECL Ultra  Stabilized Peroxide Reagent (B) 按體積比例 1:1 混合 注:為了獲得最佳效果,使用前準備稀釋液并立即使用。吸取 NcmECL Ultra 不同 組份時要換移液管,以免污染試劑。 2. 從 TBST 或 PBST 緩沖液的托盤中取出膜,小心移去膜上的多余緩沖液,但不要讓膜干燥。 3. 將膜上有蛋白的一面朝上平整地鋪在一張紙板上,加上配好的工作稀釋液。用量以覆  蓋膜為基準,1ml 工作液可以覆蓋大約 10cm2的膜。 4. 將膜與工作稀釋液孵育 1-5min,以確保整個表面覆蓋。 5. 通過放射自顯影膠片或成像設備獲取信號。對于一個未知的信號,可調節不同曝光的  時間來獲得最佳結果。

保存條件

4℃保存。

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